Yes, if your iMac is (late 2012) or newer.

Please always specify the exact model or year of manufacture for Apple products so that compatibility can be checked and confirmed.

According to the manufacturer's website, the Apple SuperDrive is compatible with the following products:

MacBook Pro with Retina Display
MacBook Air
iMac (late 2012) and newer
Mac mini (late 2009) and newer
Mac Pro (late 2013)